INTRODUCTION
The prevalence of abdominal aortic calcification (AAC) increases with age and is similarly distributed by gender in the general population.[
1,
2] AAC is also highly prevalent among subjects with established cardiovascular risk factors and correlates with subclinical atherosclerosis in other vascular beds.[
3-
5] The characteristic morphological trait of AAC is the calcification in the tunica media resulting from differentiation of vascular smooth muscle cells into osteoblast-like cells.[
6]
Longitudinal studies have consistently reported that AAC detected by dual energy X-ray absorptiometry (DXA), computed tomography (CT), or lateral lumbar radiographs (X-rays) is a strong predictor of cardiovascular events and mortality, independently of traditional cardiovascular risk factors.[
7-
13] Leow et al. [
1], in a recent systematic review and meta-analysis of observational studies with a higher prevalence of chronic kidney disease, demonstrated that subjects with any or more advanced AAC had 1.8- and 1.9-fold excess risk of cardiovascular and all-cause mortality than those without or less severe AAC, respectively.
Previous studies have also shown that AAC detected by X-rays or CT scan significantly increase the risk of all-cause mortality.[
9-
13] Lewis et al. [
7] originally described that Australian older women with high AAC scores detected on lateral spine DXA scans had a 1.5 higher risk of all-cause mortality than their counterparts with low AAC scores. Despite this evidence, there is scarce data regarding the association between AAC detected on lateral lumbar spine DXA scans and the risk of mortality in the general population. Given the aging of the population, the instant vertebral assessment (IVA) lateral lumbar spine scans may simultaneously detect vertebral fractures and identify individuals with subclinical atherosclerosis at potential risk of mortality. Therefore, the present observational study aimed to examine the association between the extent of AAC identified on lateral lumbar spine DXA scans and the risk of all-cause and cardiovascular mortality in a nationwide representative sample of middle-aged and older adults.
RESULTS
A total of 2,962 participants with a mean age of 57.4±standard error (SE) 0.21 years comprised the study sample. The overall crude prevalence of AAC was 28.7%±SE 1.0 and 9.4%±SE 0.6 of participants had evidence of extensive AAC. As shown in
Table 1, participants with AAC 24 scores ≥6 tended to be older, non-Hispanic white, physically inactive, lower BMI, and decreased eGFR. Similarly, diabetes, hypertension, and cardiovascular diseases were more prevalent among participants with AAC-24 scores ≥6. During a median follow-up of 69.9 months, a total of 252 (8.5%) participants died. Of those, 86 (33.3%) deaths were reported from cardiovascular diseases.
As shown in
Figure 1, Kaplan-Meir curves showed that the probability of survival in participants with AAC-24 scores ≥6 was significantly decreased compared with their counterparts with lower AAC-24 scores. In addition, it appears that survival across AAC-24 scores diverged about 40 months into the study. Although less accentuated, the probability of survival from cardiovascular was also decreased with higher AAC-24 scores (
Fig. 2).
Table 2 shows the association between AAC-24 scores and the risk of all-cause and cardiovascular mortality in adults. After adjusting for age, sex, race/ethnicity, education, and BMI, the risk of all-cause mortality was 2-fold higher among participants with AAC-24 ≥6 score than those with AAC-24 scores 0-1 (HR, 2.12; 95% CI, 1.40-3.22;
P<0.0001). This association was mildly attenuated after adjusting for lifestyle characteristics, prevalent cardiovascular risk factors, and eGFR (HR, 1.75; 95% CI, 1.13-2.71). Conversely, a non-significant slight increase in cardiovascular mortality was seen among subjects with AAC-24 score ≥6 (HR, 1.17; 95% CI, 0.60-2.30).
Table 3 shows the association between AAC-24 scores and HR for all-cause mortality according to age groups and gender. In general, AAC-24 scores were similarly distributed by gender, but significantly differed by age groups. Notably, 82.1% of the deaths occurred among participants aged ≥60 years. After adjusting for potential confounders, older adults and women with AAC-24 scores ≥6 were 2.8 (HR, 2.88; 95% CI, 1.79-4.63) and 2.4 times (HR, 2.44; 95% CI, 1.23-4.83) more likely to die than their counterparts with AAC-24 scores 0-1, respectively.
As shown in
Figure 3, the AUC of AAC-24 scores for predicting all-cause mortality were 0.67 (95% CI, 0.63-0.70) in men and 0.69 (95% CI, 0.64-0.74) in women. Similarly, as shown in
Figure 4, the AUC for predicting cardiovascular mortality was 0.68 (95% CI, 0.60-0.76) in men and 0.70 (95% CI, 0.61-0.79) in women for predicting cardiovascular mortality, corresponding to AUC with acceptable to good discriminating capacity.
DISCUSSION
The present findings indicate that adults aged 40 years and older with baseline AAC-24 scores ≥6 detected on the vertebral assessment lateral spine DXA scans and after a median follow-up of 69.9 months had 1.7-fold higher risk of all-cause mortality than their counterparts with AAC-24 scores 0-1. Notably, the risk of all-cause mortality in older adults and women with AAC-24 scores ≥6 was 2.8- and 2.4-fold higher than their counterparts with AAC-24 scores 0-1, respectively.
Previously, Lewis et al. [
7] reported that older Australian women with a baseline AAC-24 scores ≥6 detected on lumbar spine DXA scans and after a mean follow-up of 14.5 years had 1.5-fold higher risk of all-cause mortality than those with AAC-24 score 0-1, which is consistent with the present results. Likewise, in the Study of Osteoporotic Fractures, white women with AAC identified by lumbar spine X-ray and a mean follow-up of 13 years had 37% higher risk of all-cause mortality.[
10] Moreover, a systematic review and meta-analysis of population-based studies reported that subjects with any or more advanced AAC had a 1.9-fold higher absolute and relative risk for all-cause mortality than their counterparts with no or less advanced AAC.[
1]
Studies conducted predominantly in older women have demonstrated a significant association between the extent of AAC detected on lateral lumbar spine DXA scans and the incidence of myocardial infarction and stroke.[
7,
8] As expected, the present results indicate that participants with more advanced AAC had a higher prevalence of self-reported cardiovascular conditions, diabetes, hypertension. Moreover, the probability of cardiovascular survival among individuals with AAC-24 score ≥6 was considerably lower than those with lower AAC-24 scores. However, after adjusting for potential confounders, including traditional cardiovascular risk factors, cardiovascular mortality did not significantly differ according to AAC-24 scores. Likewise, Rodondi et al. [
10] reported that older women with prevalent AAC detected on lumbar spine X-ray did not have a significant risk of cardiovascular mortality, which is consistent with the present findings.
Previously, Lewis et al. [
7] demonstrated that older women with AAC-24 scores ≥6 detected on lumbar spine DXA scans had a 1.8-fold higher risk of atherosclerotic vascular mortality than those with AAC-24 scores 0-1. Likewise, in the Multiethnic Study of Atherosclerosis, participants grouped in the highest AAC quartile detected on CT scans had 5.9-fold increased risk of cardiovascular mortality compared with their counterparts in the lowest quartile.[
11] Moreover, participants in the Framingham Heart Study with the highest tertile of AAC detected on lumbar spine X-ray at baseline examination and followed over 22 years were 2.2 times more likely to die from cardiovascular diseases than those in the lowest tertile, which contrasts with the present findings.[
13] The characteristics of the participants from the present and previous studies and their all-cause and cardiovascular risk associated with AAC were summarized in
Table 4.
Possible explanations for these contradictory results regarding the association between AAC-24 scores and cardiovascular mortality might be related to the radiographic technique used to detect AAC, characteristics of the participants, follow-up study period, and atherosclerotic-related cardiovascular deaths as study outcomes. Nevertheless, a meta-analysis of three longitudinal observational studies with a least 2 years of follow-up, originally demonstrated that the risk of cardiovascular mortality was 1.7-fold higher among individuals with baseline AAC.[
21] A recent systematic review and meta-analysis of five cohort studies also reported that subjects with any or more advanced AAC had a 1.8-fold higher risk of fatal cardiovascular events.[
1]
Notably, women with AAC-24 score ≥6 were 2.4 times more likely to die than those with AAC-24 score 0-1, despite of having a lower prevalence of cardiovascular risk factors and diabetes (data not shown). In contrast, higher AAC-24 scores in men did not significantly increase the risk of all-cause mortality. Of interest, AAC-24 scores did not significantly differ by gender in the present study. Similarly, Michos et al. [
22], in the multiethnic study of atherosclerosis previously reported that sex hormones in both genders were not significantly associated with the presence and severity of AAC. Nevertheless, the exact reasons for the observed gender disparities in mortality risk associated with prevalent AAC are unclear and merit further investigation. Notably, a large study conducted to examine trends in mortality after elective abdominal aorta aneurysm surgery in England also demonstrated that all-cause mortality at 30 days, 1 year, and 5 years were higher in women than in men. Moreover, 30-day mortality risk was increased in women regardless of the type of surgical procedure (open vs. endovascular repair).[
23]
Wong et al. [
4] reported that AAC detected by CT scans was correlated with subclinical cardiovascular disease of the coronary, carotids, and leg arteries. The severity AAC was also associated with an increased likelihood of coronary artery calcification and carotid intima medial thickening. AAC-24 scores identified on lumbar spine DXA scans were also significantly correlated with carotid ultrasound measures of atherosclerosis in older women.[
5] These findings were also reported in autopsy specimens of cadavers with gross calcification of the abdominal aorta who had concomitantly lumbar spine X-ray films. Indeed, AAC identified on X-ray was 100% correlated with post-mortem abdominal aorta calcification. In contrast, among cadavers without lumbar spine X-ray calcification, only 16% of abdominal aorta specimens demonstrated calcification. In addition, in specimens of abdominal aorta with calcification, there was evidence of advanced atherosclerosis.[
24] Similarly, Vos et al. [
25], in a postmortem study conducted to validate the location of aortic calcification on CT scans reported that aortic calcifications were predominantly located in the distal abdominal aorta and atherosclerotic intimal layer. In addition, these calcifications were exclusively found in calcified fibrous cap atheroma and fibrocalcific atherosclerotic lesions.[
25]
Although there is scarce data regarding the validity of the vertebral fracture assessment lateral DXA scans to detect AAC, Schousboe et al. [
9] demonstrated that the AAC-8 score performed well on vertebral fracture images to detect postmenopausal women with a radiographic AAC-24 score of ≥5, which is associated with 2.4-fold increased risk of cardiovascular mortality. Sharif et al. [
26] also reported good level of agreement between a large collection of lateral spine images with AAC-24 scored by an experienced imaging specialist and machine learning AAC-24 scores across DXA machines from different manufactures used over the past three decades. The present findings also demonstrated that AAC-24 scores have good discriminating capacity in predicting all-cause and cardiovascular mortality in women.
This study has several limitations that should be mentioned. First, as an observational study, the causality of the association between AAC and all-cause mortality risk may not be established. Second, it is unknown whether calcifications detected by DXA scans in other segments of the aorta may increase the risk of mortality. Third, the confounder effect of low-density lipoprotein (LDL) cholesterol on the association of AAC and mortality was not examined. Nevertheless, data from the UK Biobank imaging cohort demonstrated that AAC was not correlated with LDL cholesterol.[
2] Despite these limitations, the present findings may be generalized to the U.S. adults aged 40 years and older.
In conclusion, the extent of AAC detected on lateral lumbar spine DXA scans among U.S. adults was associated with an increased risk of all-cause mortality, particularly among older adults and women. The present findings underscore the importance of routinely evaluate the presence of AAC on lateral lumbar spine DXA scans to identify subjects with subclinical atherosclerosis and concurrently implement cardiovascular risk stratification.