Korean J Bone Metab > Volume 17(2); 2010 > Article
Korean Journal of Bone Metabolism 2010;17(2):75-85.
Effect of Transforming Growth Factor-β and Bone Morphogenetic Protein on the Osteoclastogenesis
Transforming Growth Factor-β와 Bone Morphogenetic Protein이 파골세포 형성에 미치는 효과
이호승,장재석,이춘성,최영낙,차유철1*
울산대학교 의과대학 서울 아산병원 정형외과학교실, 인산병원 정형외과1
Abstract
Objective
Transforming growth factor-β (TGF-β) superfamily members, including bone morphogenetic protein (BMP) are believed to have a strong stimulatory effect on bone formation. Recently, several studies revealed that these two factors have effect on the bone resorption, but the results are controversial. Thus, in this study, we examined the effect of TGF-β1 and BMP-2 on the osteoclastogenesis.
Methods
Bone marrow cells prepared from the femur and tibia of C57BL/6 mice and non-adherent cells were used as bone marrow cell. Freshly separated bone marrow cells were cultured for 8 days in culture media including 1α, 25-(OH)2D3. After 2 or 4 days culture, TGF-β1 or BMP-2 was added with or without receptor activator of nuclear factor kappa B ligand (RANKL). The activity of tartrate resistant acid phosphatase (TRAP) was measured and the level of receptor activator of nuclear factor kappa B (RANK) mRNA was determined by reverse transcriptase - polymerase chain reaction (RT-PCR).
Results
The TRAP activity was not enhanced by TGF-β1 or BMP-2 alone but increased markedly if RANKL was added. TRAP activity increased only when the stimulants added in the early stage of culture (2nd days). The RANK mRNA level increased in TGF-β1 with RANKL group than RANKL or RANKL with BMP group.
Conclusions
The effect of TGF-β1 or BMP-2 on the osteoclastogenesis is minimal. But if added with RANKL, strong stimulatory effect was found on the osteoclastogenesis, especially in early stage of culture. The level of RANK mRNA was increased significantly in TGF-β1 and RANKL but not in BMP with RANKL group. [Korean Journal of Bone Metabolism, 17(2): 75-85, 2010]
Key Words: Bone morphogenetic protein, Osteoclastogenesis, Receptor activator of nuclear factor kappa B ligand


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