Korean J Bone Metab > Volume 17(2); 2010 > Article
Korean Journal of Bone Metabolism 2010;17(2):63-73.
Study for the Osteogenic Cells in Circulating Blood
순환 혈액 내의 골형성 세포에 대한 연구
박재형,정진하,김정화1,장재석1*
관동대학교 의과대학 명지병원 정형외과학교실, 울산대학교 의과대학 서울아산병원 정형외과학교실1
Abstract
Objective
Many studies have been currently underway to evaluate the treatment of bone defects using bone marrow derived-osteogenic cells, which could be obtained by invasive technique. The aim of this study is to investigate the existence of circulating osteogenic cells in peripheral blood by means of less invasive technique.
Methods
Using monocytes obtained from 20 mL peripheral blood and bone marrow in 4 cases, we performed reverse transcriptase-polymerase chain reaction (RT-PCR) with OC (osteocalcin) and ALP (alkaline phosphatase) which are osteogenic markers and ALP staining. We analysed, using flow cytometry, the relevance between age and number of osteogenic cells, and compared cell counts of fractured patients to those of diseased patients in 21 cases.
Results
We confirmed the presence of osteogenic cells in peripheral blood. There were no statistical differences in cell counts between fractured patients and diseased patients, and no relevance between age and number of osteogenic cells.
Conclusions
There are osteogenic cells in peripheral blood, but further studies carried out in large number and large amount of blood are required to prove the role of osteogenic cell in bone remodelling and fracture healing. [Korean Journal of Bone Metabolism, 17(2): 63-73, 2010]
Key Words: Flow cytometry, Osteogenic cells, Peripheral blood
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