Objective
The role of JAK on osteoclast differentiation and anti-bone resorptive activity has been recently reported but
the study in vivo is not well defined. In this study, we investigated the effects of a pan-JAK inhibitor, pyridone 6, on
osteoclast differentiation and bone-resorption and inflammatory bone loss model in vivo.
Methods
Mature osteoclasts were obtained using a coculture system from bone marrow cells and calvarial osteoblasts
with or without pyridone 6 in various concentrations. ICR mice were orally administered pyridone 6 or PBS 1 day
before the injection of LPS and every other day for 8 days. The femurs were obtained and micro-CT images, and
histologic sections were obtained with TRAP staining.
Results
Pyridone 6 inhibited osteoclast differentiation in co-cultures stimulated by LPS. Micro-CT and histologic
analysis of the femurs showed that the bone loss caused by LPS treatment was significantly attenuated in mouse
administered with pyridone 6.
Conclusion
These results demonstrate inhibition of JAK may be useful for the treatment of bone-resorptive diseases,
such as osteoporosis. [Korean Journal of Bone Metabolism, 17(1): 13-18, 2010]
