| Effect of High Extracellular Calcium and Magnesium on Osteoclast Formation
in Mouse Marrow Culture
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| 고농도의 칼슘 및 마그네슘이 파골세포의 생성에
미치는 효과에 관한 연구
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| 정호연,황창선1,강영순1,문인걸1,오한진2,최규홍3,김의현4,이득주5,황지주,임창훈,한기옥,장학철,윤현구,한인권 |
| 성균관대학교 의과대학 삼성제일병원 내과, 내분비 연구실1, 가정의학과2,
산부인과3, 대구파티마병원 내과4, 유타대학5
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| Abstract |
Background
High extracellular calcium inhibits osteoclast function and stimulates DNA synthesis and chemotactic response in osteoblastic MC3T3-E1 cells. We can speculate that osteoclastic activity is thought to be under the control of feedback regulation by extracellular calcium. The present study was performed to investigate whether the calcium-sensing receptor (CaR) was expressed in osteoclast-like cells from mouse marrow cells and to identify whether extracellular calcium regulated the gene expression in osteoblasts.
Method: We performed mouse bone marrow cultures for osteoclast formation in high concentration of extracellular calcium and magnesium. RT-PCR for the estimation of mRNA expression of osteoprotegerin in osteoblasts was performed in cultured ST2 cell in various concentraion of calcium and magnesium. To identify the presence of CaR in osteoclasts, we did RT-PCR and immunohistochemistry for CaR.
Results
High concentration of extracellular calcium and magnesium dependently inhibited 1,25(OH)2D3-induced osteoclast-like cell formation in mouse marrow culture. The use of RT-PCR with CaR specific primers identified CaR transcript in isolated osteoclast-like cells. Immunohistochemistry using polyclonal anti-CaR antisera also detected CaR in osteoclast-like cells. We could find out extracellular calcium and magnesium induced the stimulation of the expression of osteoprotegerin (OPG) mRNA in ST2 cells, even it was not statistically significant.
Conclusion
These results suggest that high extracellular calcium is involved in the regulation of osteoclastogenesis. CaR might mediate these action of calcium. |
| Key Words:
Calcium, CaR, OPG |
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