| The Effects of Growth Factors and Vitamin D on the Proliferation and
Differentiation of Human Bone Marrow Stromal Cells to
Osteoblast following Bone Marrow Transplantation
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| 성장인자와 비타민 D가 골수이식 후 인체 골수기질세포로부터
조골세포로의 증식 및 분화에 미치는 영향
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| 오은숙1,오기원1,이원영2,백기현,김혜수,한제호,이광우,손호영,강성구,김춘추,강무일 |
| 가톨릭대학교 의과대학 내과학교실, 미즈메디병원 내과1, 성균관대학교 강북삼성병원 내과2 |
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| Abstract |
Background
Bone marrow stromal cells (BMSCs) are believed to play a major role in bone formation as a major source of osteoprogenitor cells. However, very little is known about how the osteogenic differentiation of these cells is regulated by systemic hormones and local growth factors. Fibroblast growth factor (FGF-2), Insulin-like growth factors (IGFs) and 1,25-dihydroxyvitamin D3 are all important bone regulatory factors known to affect proliferation and differentiation of human osteoblasts. We have investigated the effects of FGF-2, IGF-1 and 1,25-dihydroxyvitamin D3 on the proliferation and differentiation of human BMSCs in ex vivo culture and also tried to identify the effect of those agents on BMSCs of normal people and the patients undergoing bone marrow transplantation (BMT).
Methods
Human BMSCs from 11 normal people and 6 patient undergoing BMT were expanded for 14 days and then subcultured. The effect of FGF-2, IGF-1 or 1,25-dihydroxyvitamin D3 on the number and mean area of colonies was assessed by crystal-violet stain, and the differentiation was assessed by calcium assay during primary culture. We also measured the proliferation by MTT assay, and the differentiation to osteoblast by measuring the alkaline phosphatase (ALP) activity and expression of mRNA of osteocalcin (OC) during secondary culture.
Results
FGF-2 treatment increased the mean area of colonies in both BMSCs of normal people and the patients undergoing BMT during primary culture. 10 ng/ml concentration of FGF-2 decreased the ALP activity in both groups during secondary culture. 1,25-dihydroxyvitamin D3 enhanced the ALP activity and the expression of osteocalcin mRNA during secondary culture in both groups. Treatment with IGF-1 had no detectable effect on the proliferation and the differentiation of BMSCs.
Conclusion
FGF-2 increased the proliferation of human BMSC-derived osteoblast during primary culture, and whereas it significantly decreased the amount of differentiation during se |
| Key Words:
Human bone marrow stromal cell, Fibroblast growth factor-2, Insulin-like growth factor-1, 1, 25-dihydroxyvitamin D3, Proliferation, Differentiation |
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