| The Effect of gp130 on Osteoclastic Differentiation and Activation |
| gp130 유전자가 파골세포 분화 및 활성에 미치는 영향 |
| 신홍인,김인옥,조현민,김신윤**,류현모* |
| 경북대학교 치과대학 구강병리학교실, 구강생화학교실*, 경북대학교 의과대학 정형외과학교실** |
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| Abstract |
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Signals through gp130 induce diverse biological effects in immune, hematopoietic, and nervous systems. Substantial evidence also indicates that the IL-6 related cytokines have profound effects on the bone metabolism by regulating both osteoclastic and osteoblastic development and function. However, their mechanisms were not well elucidated. To understand the effects of gp130-mediated signals on osteoclastic differentiation and activation, we investigated the developing skeleton of gp130 deficient mice by skeletal preparation with histological examination focusing on tibia through routine H&E and von Kossa staining. The TRAP activity was also detected histochemically. The expression pattern of osteoclast marker genes, TRAP and MMP-9 mRNAs, was evaluated by in situ hybridization. gp130 deficient mice revealed retarded skeletal development with bending of long bones of digits. Histologically they showed poor calcified matrix formation with marked reduction of primary spongiosa and concentration of numerous large round shaped multinucleated TRAP (+) cells along the border of growth plate. The gp130 deficient osteoclasts demonstrated poor development of ruffled border in electron microscopic observation. These osteoclasts expressed higher TRAP mRNA signals compared to wild type osteoclasts whereas MMP-9 expression in osteoclasts located at diaphysis of gp130 deficient mice was reduced compared to that of wild type osteoclasts. These finding suggest that the gp130-mediated signals may important for full activation of osteoclasts rather than osteoclastic differentiation. |
| Key Words:
gp130, Osteoclasts, in situ hybridization |
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