The Effects of C161→T Polymorphisms in Exon 6 of Peroxisome Proliferator-Activated
Receptor-γ Gene on Bone Mineral Metabolism and Serum Osteoprotegerin Levels
in Perimenopausal Korean Women
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한국인 여성에서 Peroxisome Proliferator-Activated Receptor-γ
Gene Exon 6 C161→T 유전자 다형성이 폐경 전후 골대사와
혈중 Osteoprotegerin 농도에 미치는 효과
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이은정1,이원영1,김세연2,오은숙3,백기현4,오기원5,박경창5,한기옥6,윤현구6,강무일4,김선우1 |
성균관대학교 의과대학 강북삼성병원 내과1, 강북삼성병원 의과학 연구소2, 미즈메디병원 내과3,
가톨릭대학교 의과대학 내과4, 한림대학교 의과대학 내과5, 삼성제일병원 내과6
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Abstract |
Background Peroxisome proliferator-activated receptor (PPAR)γ is mainly known to be involved in adipocyte differentiation. Recent studies revealed the inhibitory role of PPARγ in osteoblastogenesis and suggested the possibility as the candidate gene for osteoporosis. We observed the frequency of C161→T substitution in exon 6 of PPARγ in Korean females and examined the association of different genotypes and the menopausal status with bone turnover markers, bone mineral density (BMD) and serum osteoprotegerin (OPG), which plays an inhibitory role in osteoclastogenesis.
Methods In 263 healthy Korean women (mean age 51.5±6.8 yrs; range 37~73 yrs), anthrophometric measurements were done and lumbar spine and femoral neck BMD, bone turnover markers, such as alkaline phosphatase (ALP), serum calcium, phosphorus, 24 hrs urine calcium, phosphorus excretion and urine deoxypyridinoline were measured. Serum follicular stimulating hormone (FSH) levels were measured and serum OPG levels were measured with sandwich enzyme-linked immunosorbent assay (ELISA) method. DNAs were extracted from the samples and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was performed and the sequencing of the products was done to confirm the substitution.
Results Allele frequencies were 0.804 for C allele and 0.196 for T allele, which was in Hardy-Weinberg equilibrium (p=0.94). There were no differences in mean age, body mass index (BMI), BMD and bone turnover markers among different genotypes and subjects with T alleles showed significantly lower serum OPG levels (p=0.003). There were no differences in the prevalences of osteoporosis according to the genotypes. Analyzed according to the menopausal status, only in postmenopausal women, subjects with T alleles had significantly lower serum OPG levels, which showed consistently significant differences after adjustment for age and FSH (p<0.05). There were no differences in mean values for other variables among dif |
Key Words:
PPARγ, Bone mineral density, Osteoprotegeri |
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