| Effect of TGF-β1 and BMP-2 for the Chondrogenesis of
Human Bone Marrow Stem Cells
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| TGF-β와 BMP-2가 골수 줄기세포의 연골세포 분화에
미치는 영향에 관한 연구
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| 윤경호 |
| 경희대학교 의과대학 정형외과학교실 |
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| Abstract |
Purpose
The aim of this study was to determine the effect of TGF-β1 or BMP-2 on the chondrogenesis of adult human bone marrow stem cells.
Material and Methods: Human bone marrow stem cells were isolated from marrow aspirates of iliac crest, and adherent cell cultures were established. The cells were subsequently exposed to 0, 10, 50, 100 ng/ml of BMP-2 and 1, 5, 10 ng/ml of TGF-β1 in a serum-free medium to make 7 groups. The chondrogenesis of culture products was then assessed in terms of glycosaminoglycan (GAG) production with alcian blue staining and dimethylmethylene blue dye assay, total collagen with sirius red dye assay, and type II collagen with immunocytochemistry. We performed 6 repeated independent experiments.
Results
Treatment with 100 ng/ml BMP-2 significantly increased the formation of GAG and total collagen, but did not increase type II collagen production (p>0.05). In cultures treated with 10 ng/ml TGF-β1, there was a significant increase in the production of GAG, total collagen, and type II collagen (p<0.05).
Conclusion
Our results suggest that 10 ng/ml TGF-β1 has a stimulatory effect on the chondrogenesis of adult human bone marrow stem cells. Further studies must be conducted to growth factors or other elements to stimulate chondrogenesis in vitro or in vivo.
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| Key Words:
Bone marrow stem cells, Chondrogenesis, TGF-β1, BMP-2 |
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